Coding
Part:BBa_K4115000:Design
Designed by: Kaijun Wang Group: iGEM22_ShanghaiTech_China (2022-09-19)
Superfolder GFP (mammalian codon optimized)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
It can be used as a reporter. For example, you can add it downstream an uncharacterized promoter.By measuring the fluorescence intensity, you can know the relative strength of the promoter.
Source
This part is amplified from pET21a-sfGFP with PCR. pET21a-sfGFP is a gift from Professor Hisashi Tadakuma'lab of Shanghaitech University.