Coding

Part:BBa_K4115000:Design

Designed by: Kaijun Wang   Group: iGEM22_ShanghaiTech_China   (2022-09-19)


Superfolder GFP (mammalian codon optimized)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It can be used as a reporter. For example, you can add it downstream an uncharacterized promoter.By measuring the fluorescence intensity, you can know the relative strength of the promoter.


Source

This part is amplified from pET21a-sfGFP with PCR. pET21a-sfGFP is a gift from Professor Hisashi Tadakuma'lab of Shanghaitech University.

References